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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP502173VEZYeast1mgQuote
EP502173VEZE. coli1mgQuote
BP502173VEZBaculovirus200ugQuote
MP502173VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LVL9
Gene NamephnW; Locus:VCM66_A0562
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-367
Expression Tag6xHis
Purity>90%
AA SequenceMKNAYLLLTPGPLSTSESVREAMLKDWCTWDDDYNLEIVEVIRRKLVTLATTQSGYTSVL MQGSGTASVEATIGSVMLPTDKLLVIDNGAYGARIAQIAQYLNIACRVIAPGETAQPNLD EIADVLTHDPAITHVAIVHCETTTGMLNPIAEVAKIAKQHGKRVILDAMSSFGGIPMDIG ALGIDFMISSANKCIQGVPGFGFVIAKRSELEQCQGRARSLTLDLFDQWQCMEKNHGKWR FTSPTHTVRAFYQALLELESEGGIAARYQRYQTNQTQLVKGMRELGFAPLLPEKLHSPII TSFYSPEHSDYQFAEFYQRLKQQGFVIYPGKVSHADCFRIGNIGEVYPQDIERLLSAMQH AIYWQQA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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