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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501602BQGYeast1mgQuote
EP501602BQGE. coli1mgQuote
BP501602BQGBaculovirus200ugQuote
MP501602BQGMammalian Cell200ugQuote

Protein Information

SpeciesBacillus anthracis (strain CDC 684 / NRRL 3495)
UniProt IDC3LAM8
Gene NamephnW; Locus:BAMEG_3255
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMTENHYLLLTPGPLTTTKTVKEVMLYDWCTWDVEYNTMVQKVRAKLVSLATKEEEKYTTV LMQGSGTFSVEAVIGSVIPKNGKLLVCTNGAYGKRIVQMAEMLHIDVVVSQTEEWEPTNI VEVEKILQQDKEITHIAVVHCETTTGIINPIVDVCKLGKQYGKVTLVDAMSSFGGIEIDI AELQIDFLISSANKCIQGVPGFGFVIAQRDELLKCKGQARSLSLDLYDQWETMENQNGKW RFTSPTHVVHAFYQALLELEKEGGVRARYNRYYNNQKLLVNRMGEIGFKPLVNEKYQSPI ITSFIYPEGNFEFQQLYNELKRYGFVIYPGKISKVDTFRIGNIGDVHEEDINRLVDSIAK GVVIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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