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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP497634BQQYeast1mgQuote
EP497634BQQE. coli1mgQuote
BP497634BQQBaculovirus200ugQuote
MP497634BQQMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain Q1)
UniProt IDB9IUR6
Gene NamephnW; Locus:BCQ_1399
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMTENHYLLLTPGPLTTTKTVKEVMLYDWCTWDVEYNMMVQQVRAKLVSLATKEEERYTTV LMQGSGTFSVEAVIGSVIPKNGKMLVCTNGAYGKRIVQMAEMLHIDVVVSQTEEWEPTNI VEVEKILQQDKEITHIAVVHCETTTGIINPIVDVCKLGKQYGKVTLVDAMSSFGGIEIDI AELQIDFLISSANKCIQGVPGFGFVIAKRDELLKCKGQARSLSLDLYDQWETMENQNGKW RFTSPTHVVHAFYQALLELEKEGGVKARYNRYDNNQKLLVNRMREIGFKPLVDEKYQSPI ITSFIYPEEWFDFEQLYNELKRDGFVIYPGKISKVDTFRIGNIGDVHEADIHRLVDSIAK GVVIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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