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Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW)

Recombinant 2-aminoethylphosphonate--pyruvate transaminase (phnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP434170BONYeast1mgQuote
EP434170BONE. coli1mgQuote
BP434170BONBaculovirus200ugQuote
MP434170BONMammalian Cell200ugQuote

Protein Information

SpeciesBacillus weihenstephanensis (strain KBAB4)
UniProt IDA9VKQ3
Gene NamephnW; Locus:BcerKBAB4_1241
Protein Name2-aminoethylphosphonate--pyruvate transaminase
Region Expressed1-365
Expression Tag6xHis
Purity>90%
AA SequenceMNENHYLLLTPGPLTTTKTVKEVMLYDWCTWDVEYNTMVQDVRNRLVSLATKEEEKYTTV LMQGSGTFSVEAVIGSVIPRKGKLLVCTNGAYGKRIVQMAEMLHIDVVVSQTEEWEPTNI AEVEQLLQQDKEITHIVVVHCETTTGIINPIVDVCKLGKKYGKVTLVDAMSSFGGIEIDI AELQIDFLISSANKCIQGVPGFGFVIANRDELLKCKGQARSLSLDLYDQWETMEEQNGKW RFTSPTHVVHAFYQALLELEEEGGVRARYNRYYSNQKLLVHKMKEMGFKPLVDEKYQSPI ITSFIYPEAGFEFLQLYNELKRYGFVIYPGKISKVDTFRIGNIGDVHEDDINRLVDSIAK GVVIG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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