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Recombinant 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (GCAT)

Recombinant 2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial (GCAT) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP009308HUYeast1mgQuote
EP009308HUE. coli1mgQuote
BP009308HUBaculovirus200ugQuote
MP009308HUMammalian Cell200ugQuote

Protein Information

SpeciesHomo sapiens (Human)
UniProt IDO75600
Gene NameGCAT; aka: KBL
Protein Name2-amino-3-ketobutyrate coenzyme A ligase, mitochondrial
Region Expressed22-419
Expression Tag6xHis
Purity>90%
AA SequenceSALAQLRGILEGELEGIRGAGTWKSERVITSRQGPHIRVDGVSGGILNFCANNYLGLSSH PEVIQAGLQALEEFGAGLSSVRFICGTQSIHKNLEAKIARFHQREDAILYPSCYDANAGL FEALLTPEDAVLSDELNHASIIDGIRLCKAHKYRYRHLDMADLEAKLQEAQKHRLRLVAT DGAFSMDGDIAPLQEICCLASRYGALVFMDECHATGFLGPTGRGTDELLGVMDQVTIINS TLGKALGGASGGYTTGPGPLVSLLRQRARPYLFSNSLPPAVVGCASKALDLLMGSNTIVQ SMAAKTQRFRSKMEAAGFTISGASHPICPVMLGDARLASRMADDMLKRGIFVIGFSYPVV PKGKARIRVQISAVHSEEDIDRCVEAFVEVGRLHGALP
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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