Request QuoteCatalog Number: xP006462MOSize: 0.2-1mg

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Recombinant 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (Cyp8b1)

Recombinant 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase (Cyp8b1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP006462MOYeast1mgQuote
EP006462MOE. coli1mgQuote
BP006462MOBaculovirus200ugQuote
MP006462MOMammalian Cell200ugQuote

Protein Information

SpeciesMus musculus (Mouse)
UniProt IDO88962
Gene NameCyp8b1; aka: Cyp12
Protein Name7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase
Region Expressed1-500
Expression Tag6xHis
Purity>90%
AA SequenceMTLWCTVLGALLTVVGCLCLSLLLRHRRPWEPPLDKGFVPWLGHSMAFRKNMFEFLKGMR AKHGDVFTVQLGGQYFTFVMDPLSFGPIIKNTEKALDFQSYAKELVLKVFGYQSVDGDHR MIHLASTKHLMGQGLEELNQAMLDSLSLVMLGPKGSSLGASSWCEDGLFHFCYRILFKAG FLSLFGYTKDKQQDLDEADELFRKFRRFDFLFPRFVYSLLGPREWVEVSQLQRLFHQRLS VEQNLEKDGISCWLGYMLQFLREQGIASSMQDKFNFMMLWASQGNTGPTCFWVLLFLLKH QDAMKAVREEATRVMGKARLEAKKSFTFTPSALKHTPVLDSVMEESLRLCATPTLLRVVQ EDYVLKMASGQEYQIRRGDKVALFPYLSVHMDPDIHPEPTAFKYDRFLNPDGTRKVDFYK SGKKIHHYSMPWGSGVSKCPGRFFALSEMKTFVLLMIMYFDFKLVDPDIPVPPIDPRRWG FGTSQPSHEVRFLYRLKPVQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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