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Recombinant Alpha-fetoprotein (AFP)

Recombinant Alpha-fetoprotein (AFP) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001421BOYeast1mgQuote
EP001421BOE. coli1mgRPA153Bo01
BP001421BOBaculovirus200ugQuote
MP001421BOMammalian Cell200ugQuote

Protein Information

SpeciesBos taurus (Bovine)
UniProt IDQ3SZ57
Gene NameAFP
Protein NameAlpha-fetoprotein
Region Expressed19-610
Expression Tag6xHis
Purity>90%
AA SequenceRTMHKNAYGIDSILDSSPCSSGTNLVGLATIFFAQSVQGATYEEVSQMVKDVLTIIEKPT GSKQPAGCLENQVSAFLEEICREKEIPEKYGLSDCCSRTGEERHDCFLAHKKAAPASIPP FPVLEPVTSCKSYKENRELFINRYIYEIARRHPVLYAPTILSVANQYNKIIPHCCKAENA TECFETKVTSITKELRESSLLNQHICAVMGKFGPRTFRAITVTKVSQKFPKANFTEIQKL VMDVAHIHEECCKGNVLECLQDGERVMSYICSQQDILSRQIAECCKLPTTLELGHCIIHA ENDDKPEGLSPNVNRFLGDRDFNQLSSRDKDLSMARFTYEYSRRHTKLAVPIILRVAKGY QELLEKCSQSENPSECQDKGEEELEKYIQESQALAKRSCGLFQKLGEYYLQNAFLVAYTK KAPQLTSPELMALTRKMANAGAICCHLSEDKQLACGEGVADLIIGHLCIRHEENPINPGV DQCCTSSYSNRRPCFSSLVVDETYVPPPFSDDKFIFHKDLCQVQGVPLQTMKQQFLINLV KQKPQITEEQLETVVADFSGLLEKCCQSQEQEVCFTEEGPALISKTRAALGV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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