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Recombinant Alanine--tRNA ligase, mitochondrial (Aars2)

Recombinant Alanine--tRNA ligase, mitochondrial (Aars2) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001024RAYeast1mgQuote
EP001024RAE. coli1mgQuote
BP001024RABaculovirus200ugQuote
MP001024RAMammalian Cell200ugQuote

Protein Information

SpeciesRattus norvegicus (Rat)
UniProt IDD3ZX08
Gene NameAars2; ORFs:rCG_43500
Protein NameAlanine--tRNA ligase, mitochondrial
Region Expressed24-985
Expression Tag6xHis
Purity>90%
AA SequenceRFSTEPHPPHGAAVRDAFLSFFRDRHGHRLVPSASVRPRGDPSLLFVNAGMNQFKPIFLG TVDPRSEMAGFRRVANSQKCVRAGGRHNDLEDVGRDLSHHTFFEMLGNWAFGGEYFKKEA CSMAWELLTQVYGIPEDRLWVSYFSGDSKTGLDPDLETRDIWLSLGVPASRVLSFGLQEN FWEMGDTGPCGPCTEIHYDLAGGMGPPQLVELWNLVFMQHYREADGSLHLLPQQHVDTGM GLERLVAVLQGKHSTYDTDLFSPLLDAIHQSCRVPPYSGRVGAADEGRIDTAYRVVADHI RTLSVCIADGVSPGMSGAPLVLRRILRRAVRYSTEVLQAPPGFLGNLVPVVVATLGAAYP ELQKNSVKVLIWEIANLVSEDEAAFLASLQRGRRIIDRTVKRLGPSDLFPAEVAWSLSLS GNLGIPLDLVQLMLEEKGVKLDTAGLEQLAQKEAQHRAQQAEAAQEEGLCLDVHALEELH RQGIPTTDDSPKYNYSLRPNGDYEFGLCEAQVLQLYSETGTAVASVGEGQRCGLLLDRTN FYAEQGGQASDRGYLIRTGQQDVLFPVARAQVCGGFILHEAMAPECLQVGDRVQLYVDKA WRMGCMVKHTATHLLNWALRQTLGPTTEQRGSHLNPERLRFDVATQTPLTTEQLRTVESY VQEAVGQDKPVYMEEVPLAHTARIPGLRSLDEVYPDPVRVVSVGVPVAQALAPASQAALQ TSVELCCGTHLLSTGAVGDLVIIGDRQLVKGITRLLAITGEQAQQAREVGQSLSQEVEVA SERLSRGSRDLLEAHRLSKDIGRLIEFTESAVIPQWQRQEQQTTLKMLQRRANTAIRKLE KSQATEKSQELLKRHSEGPLIVDTVSAQSLSVLVKVVRQLCKQAPSMSVLLLSPQPTGSV LCACQVAQGATPTFTAEAWALAVCSHMGGKAWGSPVIAQGTGHTADLEAALRTARAYALN QL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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