Stimulates growth of bovine capillary endothelial cells by 3-5 fold over 5% calf serum at 10-25ng/ml FGF and 0ug/ml heparin.
Each 5ug aFGF were lyophilized from 0.5ml solution containing 1mM sodium phosphate, pH 7 after filtration over a low binding membrane.
Sterile Filtered White lyophilized (freeze-dried) powder.
It is recommended to reconstitute the lyophilized aFGF in sterile 50mM Na2HPO4 pH-7, and 0.5% albumin. The Recommended concentration in cell culture: 1-20ng/ml.
Lyophilized aFGF although stable at room temperature for 2 weeks, should be stored desiccated below -18oC. Upon reconstitution aFGF should be stored at 4oC between 2-3 weeks and for future use below
-18oC.
Please prevent freeze-thaw cycles.
HBGF-1, ECGF-beta, FIBP, FGFIBP, FIBP-1, ECGF, ECGFA, GLIO703, FGF1, FGF-a.