Request QuoteCatalog Number: xP001380FHVSize: 0.2-1mg

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Recombinant ADP-dependent glucokinase (glkA)

Recombinant ADP-dependent glucokinase (glkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001380FHVYeast1mgQuote
EP001380FHVE. coli1mgQuote
BP001380FHVBaculovirus200ugQuote
MP001380FHVMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus abyssi (strain GE5 / Orsay)
UniProt IDQ9UYQ3
Gene NameglkA; Locus:PYRAB14540; ORFs:PAB0967
Protein NameADP-dependent glucokinase
Region Expressed1-452
Expression Tag6xHis
Purity>90%
AA SequenceMSWDEMYRDAYERVLNSIGKIKGVMLAYNTNIDAIKYLKREDLERRIEEAGKDEVLRYSD ELPKKINTIQQLLGSILWSVKRGKAAELLVEDREVRNYMRQWGWDELRMGGQVGIMANLL GGVYGIPVIAHVPQISKLQASLFLDGPIYVPTFEEGLKLVHPRNFEGNEEDCIHYIYEFP RGFKVLNFTAPRENRFIGAADDYNPRLYIRKEWVERFEEIAERAELAIVSGLHSLTEETY REPIKVVREHLKVLKDLNIKTHLEFAFTADEKVRREILGLLSLVYSVGLNEVELASVLEI MNERELADRILAKDPADPVAVIEGLMKLIEEGVERIHFHTYGYYLAITKYRGEHVRDALL FSALAAATKAMLGNIEKLDDLRKGLEVPIGRQGLEVYEVVKREFNVEKGIGEVGDYQIAF VPTKIVEKPKSTVGIGDTISSSAFVSEFSLSS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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