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Recombinant ADP-dependent glucokinase (glkA)

Recombinant ADP-dependent glucokinase (glkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001380TMQYeast1mgQuote
EP001380TMQE. coli1mgQuote
BP001380TMQBaculovirus200ugQuote
MP001380TMQMammalian Cell200ugQuote

Protein Information

SpeciesThermococcus litoralis
UniProt IDQ7M537
Gene NameglkA
Protein NameADP-dependent glucokinase
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMKESLKDRIRLWKRLYVNAFENALNAIPNVKGVLLAYNTNIDAIKYLDKDDLEKRVTEIG KEKVFEIIENPPEKISSIEELLGGILRSIKLGKAMEWFVESEEVRRYLREWGWDELRIGG QAGIMANLLGGVYRIPTIVHVPQNPKLQAELFVDGPIYVPVFEGNKLKLVHPKDAIAEEE ELIHYIYEFPRGFQVFDVQAPRENRFIANADDYNARVYMRREFREGFEEITRNVELAIIS GLQVLKEYYPDGTTYRDVLDRVESHLNILNRYNVKSHFEFAYTANRRVREALVELLPKFT SVGLNEVELASIMEIIGDEELAKEVLEGHIFSVIDAMNVLMDETGIERIHFHTYGYYLAL TQYRGEEVRDALLFASLAAAAKAMKGNLERIEQIRDALSVPTNERAIVLEEELEKEFTEF ENGLIDMVDRQLAFVPTKIVASPKSTVGIGDTISSSAFVSEFGMRKR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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