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Recombinant ADP-dependent glucokinase (glkA)

Recombinant ADP-dependent glucokinase (glkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001380FHYYeast1mgQuote
EP001380FHYE. coli1mgQuote
BP001380FHYBaculovirus200ugQuote
MP001380FHYMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus kodakaraensis (strain ATCC BAA-918 / JCM 12380 / KOD1) (Thermococcus kodakaraensis (strain KOD1) )
UniProt IDQ5JE39
Gene NameglkA; Locus:TK1110
Protein NameADP-dependent glucokinase
Region Expressed1-453
Expression Tag6xHis
Purity>90%
AA SequenceMNWDGLYASAFERIRDNIGKVGNVLLAYNTNIDAIKYLEREDLERRIGAAGREEVLPYSE KLPKRIESVPQLLGSILWSIRRGKAAELFVESCSTRFYMRRWGWDELRMGGQVGIMANLL GGVYGVPVIAHVPQLSRLQAELFKDGPIYVPKVEGGKLKLVHPKEFKADEENCIHYIYEF PRGFRVFDFEAPRENRFIGSADDYNTNVVIRPEFEEHFGEIAEKTELAIISGLQALTEGN YREPFETIKEHLDVLEGKGIPAHLEFAFTPDETVRKEILGVLGKFWSVGLNEVELASIMD VMGEKTLAEKLLAHDPVDPIVVTKAMLKLAEKTGVRRIHFHTYGYYLALTDYRGEFVRDA LLFAALAAAAKAKLGDVRNIDDVVKAMDVPVNEKAKGVEEALTKEYGMENGIAEVNGYQL AFIPTKIVAKPKSTVGIGDTISSSAFVGEFALR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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