Request QuoteCatalog Number: xP001380TMTSize: 0.2-1mg

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Recombinant ADP-dependent glucokinase (glkA)

Recombinant ADP-dependent glucokinase (glkA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001380TMTYeast1mgQuote
EP001380TMTE. coli1mgQuote
BP001380TMTBaculovirus200ugQuote
MP001380TMTMammalian Cell200ugQuote

Protein Information

SpeciesThermococcus sibiricus (strain MM 739 / DSM 12597)
UniProt IDC6A124
Gene NameglkA; Locus:TSIB_0252
Protein NameADP-dependent glucokinase
Region Expressed1-467
Expression Tag6xHis
Purity>90%
AA SequenceMKESLKERIKLWKNLYMNAFENAVNAIPNVEGVLLAYNTNIDVIKYLNKDDLERRINQIG KERVFEVIKAPTERISSLEHLFGGILRSIKLGKAIEWFVENEEIRRYLREWGWDELRIGG QAGIMANLIGGVYRIPTIVHVPQNPKLQADLFVDGPIYVPIFEGKELKLVHPKEAVKEEN ELIHYIYEFPRGFQVFDIQAPRENRFIANADDYNARVYMRKEFKESFEKIAKGVELAIIS GLQVLKEYYSDGTTYRDVLDRVESHLNILNRYGVRSHFEFAYTPNRLVREELIGILSKFT SVGLNEVELASIVEIIGDETLAKEVLEGHVFPIIDAINLLMDETGIDRIHLHTYGYYLAL TKYRSEEVRDALLFASLAAAAKAMKGNIEKIDHVKDALSVPTNERAIVFEEELEKEFTEF ENGLIDMVDRQLAFVPTKIVTSPKSTVGIGDTISSSAFVSEFAMRKS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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