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Recombinant Adenylosuccinate lyase (purB)

Recombinant Adenylosuccinate lyase (purB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001397FHXYeast1mgQuote
EP001397FHXE. coli1mgQuote
BP001397FHXBaculovirus200ugQuote
MP001397FHXMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
UniProt IDO58582
Gene NamepurB; Locus:PH0852
Protein NameAdenylosuccinate lyase
Region Expressed1-450
Expression Tag6xHis
Purity>90%
AA SequenceMAVHPIDYRYGSEEMRKIWDEKNKLQKLLDVEAALARAHAKVGNIPEESAKVISERANTK WVKLERVKEIEAEIHHDIMAVVKALSEVCGEHGKYVHLGATSNDIIDTANALLIKESLEI VERDLKELRSILKQLAEKHIGTVCIGRTHGQHAVPTTYGMKFALWLDEIQRHIERLYQLK ERVLVGKMRGAVGTAASFGEKAFEIERLVMEDLGLKPARITNQIVQRDVYAELLFFLALV ASTLDKMGLEIRNLQRTEILEVSEPFGEKQVGSSTMPHKRNPIRTEKVCGLARVLYSNVI PALLNNPLWHERDLTNSSVERVILPESFVLLDEMLKTMKKVLTGLEFFPENIRRNLYLTK NLIMAEPLMLKLAEKGMGRQEAHEVVRQLAMKAFRENRDLLEVAKESREITKYLTERDLE ELKPENYIGKAREIVKKVIEYVEEMERRGL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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