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Recombinant Adenylosuccinate lyase (purB)

Recombinant Adenylosuccinate lyase (purB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001397DOCYeast1mgQuote
EP001397DOCE. coli1mgQuote
BP001397DOCBaculovirus200ugQuote
MP001397DOCMammalian Cell200ugQuote

Protein Information

SpeciesArchaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126)
UniProt IDO28041
Gene NamepurB; Locus:AF_2242
Protein NameAdenylosuccinate lyase
Region Expressed1-444
Expression Tag6xHis
Purity>90%
AA SequenceMIVHPIDYRYGTPEMKRIWSEDSKIKRMVRVEMALLRALAKKGYLSEEEAKEAKKKAYSV TPERVKEIEAEIKHDIMALVKAITEATGCRWVHFGATSNDIIDTATALQLRDSLKILEVK IKRLAKVLADKALEYKDVVCLGRTHGQAALPTTYGFRFALWAAEVARHYIRLQQMKDRLL VGQMSGAVGTQAAFGKDGFEIEEEVMRLLNLKPALISSQIIPRDSYCEYLEFLANLAATL EKIALNFRLLQRAEVGELMEKFEAKQVGSSTMPHKRNPIDCENVCGLARVVRGFVEPQHQ SAILWEERDLTNSSAERITLVESTVLADHILTKMIKVVSSVSLNLENIRRNLEMQRGLNL SEAVMIEMTKRGVGRQEAHEILRQAAMRAYENNSSLLDELLKDERVMKYFKEDELREILK PENYLGTARERVERVVRWVNEVLK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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