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Recombinant Aconitate hydratase (acnA)

Recombinant Aconitate hydratase (acnA) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP001162MVXYeast1mgQuote
EP001162MVXE. coli1mgQuote
BP001162MVXBaculovirus200ugQuote
MP001162MVXMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium smegmatis (strain ATCC 700084 / mc (2) 155)
UniProt IDA0QX20
Gene NameacnA; Locus:MSMEG_3143
Protein NameAconitate hydratase
Region Expressed1-943
Expression Tag6xHis
Purity>90%
AA SequenceMSSENTGKSSLNSFGARDTLTVGDQSYEIYRLNAVPGTEKLPYSLKVLAENLLRTEDGAN ITKDHIEAIANWDPNAEPSIEIQFTPARVIMQDFTGVPCIVDLATMREAVAALGGDPNKV NPLAPAELVIDHSVILDVFGNASAFERNVELEYERNAERYQFLRWGQGAFDDFKVVPPGT GIVHQVNIEYLARTVMVRDGVAYPDTCVGTDSHTTMVNGLGVLGWGVGGIEAEAAMLGQP VSMLIPRVVGFKLSGEIKPGVTATDVVLTVTDMLRRHGVVGKFVEFYGKGVAEVPLANRA TLGNMSPEFGSTAAIFPIDEETINYLRLTGRTDEQLALVEAYAKAQGMWHDPEREPVFSE YLELDLSTVVPSISGPKRPQDRIELTDAKNAFRKDIHNYVEQNHPTPETKLDEAVEESFP ASDPVSLSFADDGAPDMRPSAANGATGRPTNPVLVHSEERGDFVLDHGAVVVAGITSCTN TSNPSVMLGAALLAKKAVEKGLTTKPWVKTNMAPGSQVVTDYYNKAGLWPYLEKLGYYLG GYGCTTCIGNTGPLPEEISKAINDNDLAVTAVLSGNRNFEGRISPDVKMNYLASPPLVIA YGIAGTMDFDFESDPLGQDSEGNDVFLRDIWPSAAEIEETIASSINREMFTESYADVFKG DDRWRSLPTPEGDTFEWDPASTYVRKAPYFDGMPAEPEPVSDIKGARVLALLGDSVTTDH ISPAGAIKPGTPAAQYLDANGVERKDYNSLGSRRGNHEVMIRGTFANIRLRNQLLDDVSG GYTRDFTQPGGPQAFIYDASENYKKAGIPLVVLGGKEYGSGSSRDWAAKGTVLLGVKAVI TESFERIHRSNLIGMGVIPLQFPAGESAASLKLDGTETYDIEGIEELNSGKTPKTVHVTA TKEDGSKVEFDAVVRIDTPGEADYYRNGGILQYVLRNMLKSSK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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