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Recombinant 75 kDa readthrough protein (ORF2-ORF3)

Recombinant 75 kDa readthrough protein (ORF2-ORF3) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520980BSOYeast1mgQuote
EP520980BSOE. coli1mgQuote
BP520980BSOBaculovirus200ugQuote
MP520980BSOMammalian Cell200ugQuote

Protein Information

SpeciesBeet necrotic yellow vein virus (isolate Japan/S) (BNYVV)
UniProt IDO11840
Gene NameORFs:ORF2-ORF3
Protein Name75 kDa readthrough protein
Region Expressed1-690
Expression Tag6xHis
Purity>90%
AA SequenceMSSEGRYMTWKDMSHNKFMTDRWARVSDVVSVIKQSHAMDLSKAANLSIIKTALAGLGSG WTDNNPFVSPMTRFPQTLTMYGALVLYVNLSDPEFALIMTKVSTLTDSGLADNASANVRR DVVSGNKAESSGKTAGTNENSAYTLTVSLAGLAQALRLEELMWTRDKFEDRLKLPWTPVQ GRTSPPGQXQLAAARVTAHIRAAKRALLYPGDSPEWVGWKHFYPPPPYDVYDVPPLDIIN AKLAADDIGGLVTPTPASSHGLPFEVSEEVEQANRNSLWLTVGLLLAALAVGIGVAAYHR KKLQSRLRELKLLWGSTGGSGGGGGFDTELYMRATDTVSLGTTLSEHAASAPSGLRHRPA ATDSGPHEALPFEVWVFDNLAVVYDSIGMSDLFYTVREFVGVFNGEFEGLIELLESPDDD DGVYTNAPRDTAIDAYESQENYDRIDIETVLIERRINLKKLLLEEAELERRERDMTMIAD EEQRTLLHRLESSRVEATHAVAKAEADARAAVAMAALASKEANDYDSKMAFDRSCKEQEL RLRELEVNSMPSKTERYVHTGIQGGAQLAGAMAVGAMLRRGAGSSSQTVSSGANIGSRSQ SLTRGRSASQPLSSVGGSTRGVNNNISNTNLVRAGNSAEVSAGRSTNSGNSNFWSKLRVG EGWSKYSVERAATRAQRAIVLPAPPSAPAG
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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