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Recombinant 60 kDa chaperonin (groL)

Recombinant 60 kDa chaperonin (groL) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP525390ERAYeast1mgQuote
EP525390ERAE. coli1mgQuote
BP525390ERABaculovirus200ugQuote
MP525390ERAMammalian Cell200ugQuote

Protein Information

SpeciesPantoea ananas (Erwinia uredovora)
UniProt IDO66218
Gene NamegroL; aka: groEL, mopA
Protein Name60 kDa chaperonin
Region Expressed1-541
Expression Tag6xHis
Purity>90%
AA SequenceMAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSFGAPTITKDGVSVAREI ELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQSIITEGLKAVAAGMNPMDLKRGI DQAVIAAVEELKKLSVPCSDSKAIAQVGTISANSDETVGTLIAQAMEKVGKEGVITVEEG TGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELETPFILLADKKISNIREMLPVLEAV AKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAVKAPGFGDRRKAMLQDIAILTGGTV ISEEIGMELEKAALEDLGQAKRVVINKDTTTIIDGVGEETTIQGRVTQIRQQIEEATSDY DKEKLQERVAKLAGGVAVLKVGAATEVEMKEKKARVEDALHATRAAVEEGVVAGGGVALV RVAAQLTELRGQNEDQNVGIKVALRAMESPLRQIVSNAGEEPSVVANNVKAGDGNYGYNA QTEEYGNMIDFGILDPTKVTRSALQYAASVAGLMITTECMVTDLPKGDAPDLGAGAGGMG G
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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