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Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase

Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP530055FHXYeast1mgQuote
EP530055FHXE. coli1mgQuote
BP530055FHXBaculovirus200ugQuote
MP530055FHXMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
UniProt IDO58994
Gene NameLocus:PH1259
Protein Name23S rRNA (uracil (747) -C (5) ) -methyltransferase
Region Expressed1-412
Expression Tag6xHis
Purity>90%
AA SequenceMEMRGKITKINENGLGVLGNILVPFAYPGDEVEVTETRERFGKIIARDFKLMTPSPLRIP GKCSHFGKCGGCLWQGLRYREQLKLKEEIFKRITGIEAEIKGSPRIWYFRNISNFIITVN GIGFKEFGMPKTVVNIRECPIFSERTPKYLKALKDFLRESNLKPWNWREGDVHYLQVREG KFTGEVMVNIIAHVPLNYREALMEAFNFADSIYWSLKADKKDDPRGFPTLVLGNEVIREK VEGITYLIHPSVFFQTNSYALPLLLKSVEKFCEGSKVLDLYSGIGTLSLYLAKRGFEVTG VEVNGTSVEMAKRSAEINSINATFIQGKAEDAELEGYETLIVDPPRKGLKEFSRRIVKKG PNTLIYVSCNPLRFILDYRNYLSEAYKVDDALLIDMFPHTPHIEAVIKLVRR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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