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Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase RlmC

Recombinant 23S rRNA (uracil (747) -C (5) ) -methyltransferase RlmC can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP478198ENWYeast1mgQuote
EP478198ENWE. coli1mgQuote
BP478198ENWBaculovirus200ugQuote
MP478198ENWMammalian Cell200ugQuote

Protein Information

SpeciesEscherichia coli (strain SE11)
UniProt IDB6I8H9
Gene NamerlmC; aka: rumB; Locus:ECSE_0917
Protein Name
Region Expressed1-375
Expression Tag6xHis
Purity>90%
AA SequenceMQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEWCAPVSGPEQGFRNKAK MVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALKPFIARAGLTPYNVARKRG ELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQEQLPQLKVITVNIQPVHMAIM EGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVASQLYATARDWVRQLPVKHMWDL FCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAAELGLTRLQFQALDSTQFATAQGEV PELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSCNAQTMAKDVRELPGYRIERVQLFDMF PHTAHYEVLTLLVKQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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