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Recombinant 23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD

Recombinant 23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP513007ESQYeast1mgQuote
EP513007ESQE. coli1mgQuote
BP513007ESQBaculovirus200ugQuote
MP513007ESQMammalian Cell200ugQuote

Protein Information

SpeciesPectobacterium carotovorum subsp. carotovorum (strain PC1)
UniProt IDC6DDJ9
Gene NamerlmD; aka: rumA; Locus:PC1_3390
Protein Name23S rRNA (uracil (1939) -C (5) ) -methyltransferase RlmD
Region Expressed1-449
Expression Tag6xHis
Purity>90%
AA SequenceMAQFYSPNRRVTTRKAIPAKNLTVTVTSLDPFGQGVARHEGKTVFVTGVLPGEQAEVQLT EDKRQFSHAKLKRLLTHSPQRVEPQCPHFTRCGGCQQQHADITLQQSSKTAALMRLMTRE TGAELPDASLIAGTPYAYRRRARLALYFQAKEQRLLMGYRQSNSHDLVDIKACPVLRPEL EALLQPLRDCLSRLKAVKRLGHVELVQAENGPLLVLRHLDPLHPADEQALRDFAEQQGVS VYLAPDADSLTCLHGDEPVYLVAGLTLAFSPRDFIQVNDAVNQQMVAQALAWLDVQPQDR ILDLFCGMGNFTLPLAQRAASVVGVEGVTALVEKGRENARRNALSNVTFFHQNLEDDVTQ QPWVAQGFDKILLDPARAGAAGVMEQITRLAPKRVVYVSCNATTLARDSKVLLAAGFTLV NVAMLDMFPHTGHLESMALFLHDTGTRKA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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