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Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)

Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512793VEMYeast1mgQuote
EP512793VEME. coli1mgQuote
BP512793VEMBaculovirus200ugQuote
MP512793VEMMammalian Cell200ugQuote

Protein Information

SpeciesVariovorax paradoxus (strain S110)
UniProt IDC5CLT4
Gene NamedapD; Locus:Vapar_2719
Protein Name2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Region Expressed1-277
Expression Tag6xHis
Purity>90%
AA SequenceMTQQLQQIIDAAWEDRANISSSAAPAEVRDAVEHVISELNNGKLRVATRESVGQWTVHQW IKKAVLLSFRLKDNEQMQAGSLGFYDKVPTKFSHLSANELKESGVRIVPPAVARRGSYIA KGAILMPSYVNIGAYVGEGTMVDTWATVGSCAQIGANVHLSGGVGIGGVLEPLQAGPTII EDNCFIGARSEVVEGVVIEENSVLGMGVYIGQSTPIFNRDTGETSFGRVPSGSVVISGNL PKKTKSGQEYSTYAAIIVKTVDAQTRSKTSLNDLLRD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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