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Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD)

Recombinant 2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase (dapD) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP502374RKXYeast1mgQuote
EP502374RKXE. coli1mgQuote
BP502374RKXBaculovirus200ugQuote
MP502374RKXMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium sp. (strain NGR234)
UniProt IDC3MF35
Gene NamedapD; Locus:NGR_c00680
Protein Name2,3,4,5-tetrahydropyridine-2,6-dicarboxylate N-succinyltransferase
Region Expressed1-285
Expression Tag6xHis
Purity>90%
AA SequenceMTNHDLASLSQTIETAFEDREAVSTSTRGAIRDAVEAALNLLDSGKVRVAERGADGTWTV NQWLKKAVLLSFRLNPMELVKGGPGESVWWDKVASKFDGWSVNEFEKAGFRAVPNCVVRR SAYIAPNAILMPSFVNLGAYVGEGTMVDTWATVGSCAQIGKNVHLSGGVGIGGVLEPMQA GPTIIEDNCFIGARSEVVEGCIVREGSVLGMGVFIGKSTKIVDRATGEVTYGEVPPYSVV VAGSMPSGSTMANGQPAPNLYCAVIVKRVDEKTRSKTGINELLRD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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