2,3-diketo-5-methylt-->

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Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)

Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog Number	Expression Host	Amount	In Stock
YP507027BQK	Yeast	1mg	Quote
EP507027BQK	E. coli	1mg	Quote
BP507027BQK	Baculovirus	200ug	Quote
MP507027BQK	Mammalian Cell	200ug	Quote

Protein Information

Species	Bacillus cereus (strain 03BB102)
UniProt ID	C1EQR1
Gene Name	mtnW; Locus:BCA_4149
Protein Name	2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Region Expressed	1-414
Expression Tag	6xHis
Purity	>90%
AA Sequence	MSGIIATYLIHDDSHNLEKKAEQIALGLTIGSWTHLPHLLQEQLKQHKGNVIHVEELAEH EHTNSYLRKKVKRGIIKIEYPLLNFSPDLPAILTTTFGKLSLDGEVKLIDLTFSDELKKH FPGPKFGIDGIRNLLQVHDRPLLMSIFKGMIGRNIGYLKTQLRDQAIGGVDIVKDDEILF ENALTPLTKRIVSGKEVLQSVYETYGHKTLYAVNVTGRTFDLKENAKRAVQAGADILLFN VFAYGLDVLQSLAEDDEIPVPIMAHPAVSGAYSASKLYGISSPLLLGKLLRYAGADFSLF PSPYGSVALEKEEALAISKYLTEDDVFFKKSFSVPSAGIHPGFVPFIIRDFGKDVVINAG GGIHGHPNGAQGGGKAFRTAIDATLQNKPLHEVDDINLHSALQIWGNPSHEVKL
Storage Buffer	PBS pH 7.4, 50% glycerol
Storage	Store working aliquots at 4^oC for upto a week. Freeze at -20 to -80^oC for long term storage. Avoid repeated freeze/thaw.

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