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Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)

Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP512479GFJYeast1mgQuote
EP512479GFJE. coli1mgQuote
BP512479GFJBaculovirus200ugQuote
MP512479GFJMammalian Cell200ugQuote

Protein Information

SpeciesGeobacillus sp. (strain WCH70)
UniProt IDC5D7U9
Gene NamemtnW; Locus:GWCH70_0850
Protein Name2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Region Expressed1-413
Expression Tag6xHis
Purity>90%
AA SequenceMSQVIATYLIHDEKDLEKKAEGIALGLTVGSWTDLPLLEQEQLRKHKGVVVSVQELEENE RANRYFGKRLKRGIVRIAYPSLNFSADIPALLTTVFGKLSLDGEVRLLDLTFSDEWKRKF PGPRFGIDEIRKKLDVYERPLLMSIFKGMIGRDLTYLSEQLKQQALGGVDLVKDDEILFE NELTPFEKRITAGKAVLNEVYEKTGKRTLYAVNLTGKTFELKEKAKRAVELGADVLLFNV FAYGLDVLQGLREDDEITIPIMAHPAFSGALTPSEFYGIKASLLLGKLLRLVGADFVLFP SPYGSVALDKEEALGIAAELTNDKEPFKRSFPVPSAGIHPGLVPLLFRDFGIDSIVNAGG GIHGHPDGAAGGGRAFRAAIDAALAGKSLHEAARENEALRKAIDLWGAVEVTS
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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