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Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)

Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP520012BRJYeast1mgQuote
EP520012BRJE. coli1mgQuote
BP520012BRJBaculovirus200ugQuote
MP520012BRJMammalian Cell200ugQuote

Protein Information

SpeciesBacillus subtilis (strain 168)
UniProt IDO31666
Gene NamemtnW; aka: ykrW; Locus:BSU13590
Protein Name2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Region Expressed1-405
Expression Tag6xHis
Purity>90%
AA SequenceMSELLATYLLTEPGADTEKKAEQIATGLTVGSWTDLPLVKQEQMQKHKGRVIKVEEREGT AASEKQAVITIAYPEINFSQDIPALLTTVFGKLSLDGKIKLIDLHFSEAFKRALPGPKFG VYGIRKLLGEFERPLLMSIFKGVIGRDLSDIKEQLRQQALGGVDLIKDDEIFFETGLAPF ETRIAEGKQILKETYEQTGHKTLYAVNLTGRTADLKDKARRAAELGADALLFNVFAYGLD VMQGLAEDPEIPVPIMAHPAVSGAFTSSPFYGFSHALLLGKLNRYCGADFSLFPSPYGSV ALPRADALAIHEECVREDAFNQTFAVPSAGIHPGMVPLLMRDFGIDHIINAGGGVHGHPN GAQGGGRAFRAIIDAVLEAQPIDEKAEQCKDLKLALDKWGKAEAV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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