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Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW)

Recombinant 2,3-diketo-5-methylthiopentyl-1-phosphate enolase (mtnW) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP496701BQQYeast1mgQuote
EP496701BQQE. coli1mgQuote
BP496701BQQBaculovirus200ugQuote
MP496701BQQMammalian Cell200ugQuote

Protein Information

SpeciesBacillus cereus (strain Q1)
UniProt IDB9IWP9
Gene NamemtnW; Locus:BCQ_3826
Protein Name2,3-diketo-5-methylthiopentyl-1-phosphate enolase
Region Expressed1-414
Expression Tag6xHis
Purity>90%
AA SequenceMSGIIATYLIHDDSHNLEKKAEQIALGLTIGSWTHLPHLLQEQLKQHKGNVIHVEELAEH EHTNSYLRKKVKRGIIKIEYPLLNFSPDLPAILTTTFGKLSLDGEVKLIDLTFSDELKKH FPGPKFGIDGIRNLLQVHDRPLLMSIFKGMIGRNIGYLKTQLRDQAIGGVDIVKDDEILF ENALTPLTKRIVSGKEVLQSVYETYGHKTLYAVNLTGRTFDLKENAKRAVQAGADILLFN VFSYGLDVLQSLAEDDEIPVPIMAHPAVSGAYSASKLYGVSSPLLLGKLLRYAGADFSLF PSPYGSVALEKEEALAISKYLTEDDAFFKKSFSVPSAGIHPGFVPFIARDFGKDVVINAG GGIHGHPNGAQGGGKAFRAAIDATLQNKPLHEVDDINLHSALQIWGNPSHEVKL
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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