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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (apgM) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP524504FHXYeast1mgQuote
EP524504FHXE. coli1mgQuote
BP524504FHXBaculovirus200ugQuote
MP524504FHXMammalian Cell200ugQuote

Protein Information

SpeciesPyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
UniProt IDO57742
Gene NameapgM; Locus:PH0037
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Region Expressed1-412
Expression Tag6xHis
Purity>90%
AA SequenceMVLKRKGLLIILDGLGDRPIKELNGLTPLEYANTPNMDKLAEIGILGQQDPIKPGQPAGS DTAHLSIFGYDPYETYRGRGFFEALGVGLDLSKDDLAFRVNFATLENGIITDRRAGRIST EEAHELARAIQEEVDIGVDFIFKGATGHRAVLVLKGMSRGYKVGDNDPHEAGKPPLKFSY EDEDSKKVAEILEEFVKKAQEVLEKHPINERRRKEGKPIANYLLIRGAGTYPNIPMKFTE QWKVKAAGVIAVALVKGVARAVGFDVYTPEGATGEYNTNEMAKAKKAVELLKDYDFVFLH FKPTDAAGHDNKPKLKAELIERADRMIGYILDHVDLEEVVIAITGDHSTPCEVMNHSGDP VPLLIAGGGVRTDDTKRFGEREAMKGGLGRIRGHDIVPIMMDLMNRSEKFGA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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