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Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI)

Recombinant 2,3-bisphosphoglycerate-independent phosphoglycerate mutase (gpmI) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP488378HUEYeast1mgQuote
EP488378HUEE. coli1mgQuote
BP488378HUEBaculovirus200ugQuote
MP488378HUEMammalian Cell200ugQuote

Protein Information

SpeciesHalothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
UniProt IDB8CYG0
Gene NamegpmI; Locus:Hore_15800
Protein Name2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Region Expressed1-512
Expression Tag6xHis
Purity>90%
AA SequenceMNRPKPLALIIMDGFGLSEKSDGNAIKQAHTPNLDKFFEEYPDTVLDASGEAVGLPEGQM GNSEVGHLNLGAGRIVYQDYTRINKAIEEGTLPENDALKNAIQHVKDNNSALHLMGLLSD GGVHSHINHLFGLLEMARVKNIEKVYVHPILDGRDTPPRSAEKYLNQLEEKLAELGIGEI ATVSGRYYTMDRDNRWDRTKKSYDALVLGEGLEASDAISALKQSYAADKSDEFVIPTVIK KNGQPVGTIKDNDAVIFYNFRADRARQITKALALEEFDEFERPAEHPENLYYVCMAEYDE EFNLPVAFPPLEIKNGFGEILSKEGFKQLRIAETEKYAHVTFFFNGGVEKKYPGEDRVLV PSPKVATYDEKPEMSAYEVTDKLLEKLDEDKYDVIILNFANPDMVGHTGFMDAAVKAVEA VDECLGKIIPRIVDMGGQVLLTADHGNSEKMKDSEGKPHTAHTTNQVPLIYIGGPEGVGV KPGKLADIAPTMLEILGLDTPEEMTGQSLLTR
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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