The sequence of the first five N-terminal amino acids was determined and was found to be Met-Glu-Arg-Thr-Arg.
The activity was determined by the inhibition of 4-1BB ligand mediated stimulation of IL-8 production by human PBMC. Results: 90% inhibition using 1ug for both 4-1BB ligand and 4-1BB receptor.
Lyophilized from a concentrated (1mg/ml) solution in water containing no additives.
Sterile Filtered White lyophilized (freeze-dried) powder.
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
It is recommended to reconstitute the lyophilized 4-1BB Receptor in sterile 18MΩ-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Lyophilized 4-1BB Receptor although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution 4-1BBR should be stored at 4oC between 2-7 days and for future use below -18oC. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor T-cell, antigen 4-1BB homolog, T-cell antigen ILA, CD137 antigen, CDw137, ILA, 4-1BB, MGC2172, 4-1BBR, TNFRSF9.
