Request QuoteCatalog Number: xP388498RIJSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP388498RIJYeast1mgQuote
EP388498RIJE. coli1mgQuote
BP388498RIJBaculovirus200ugQuote
MP388498RIJMammalian Cell200ugQuote

Protein Information

SpeciesRhodobacter sphaeroides (strain ATCC 17029 / ATH 2.4.9)
UniProt IDA3PIQ5
Gene NameglgB; Locus:Rsph17029_1111
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-728
Expression Tag6xHis
Purity>90%
AA SequenceMNVTSKPGELVPESDVAAIMRGTHGDPFRVLGMHGGGGAPLSVRVFAPQAAEVAVLGQGG KVLASLERIGAEGFFAGTVPGEEKFPYRLRFVSGPHEWEADDPYRFPEVLGELDEYLLGE GRHYQLYTRLGAHPAEIEGVQGVSFAVWAPNARRVSVVGAFNAWDGRRHPMRKRIGVGVW ELFVPGLHTGDLYKYEILGPSGERLPLKSDPLSFAQEAPPATASVVHGLPEAEWHDAGWM QERESRQRRDAPISIYEVHAGSWRQGLDYDALAEELSAYVREMGFTHVEFLPISEHPFTG SWGYQPIGLFAPTARFGPPEGFARLVDRLHRDGIGVILDWVPAHFPSDAHGLANFDGTHL YDHADPRQGFHRDWNTQIYNFGRQEVANFLQASALFWLDRYHVDALRVDAVASMLYLDYS RNAGEWVPNRHGGRENLEAIDFLRGVNERVRLDHPGCITIAEESTAFPQVSRPVEDGGLG FGFKWNMGWMHDTLGYFRRDPIHRKHHQNDLTFGMVYAYSEDFVLPLSHDEVVHGKGSLI GQMAGDRWQKFANLRAYFGFMWAHPGKKLLFMGGEFAQEREWNHDASLDWHLLDDPSHAG MKRLVADLNREYRKRPALHRMDCDPEGFEWIDAGDSENSVLSFLRKAPGEKPVLAVCNLT PVVRSDYRIGVPEGGEWREILNSDAAIYGGSDVGNPGGLQAEEVSWHGRPASLRLTLPPL ATIFVTPA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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