Request QuoteCatalog Number: xP385087STLSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP385087STLYeast1mgQuote
EP385087STLE. coli1mgQuote
BP385087STLBaculovirus200ugQuote
MP385087STLMammalian Cell200ugQuote

Protein Information

SpeciesShewanella baltica (strain OS155 / ATCC BAA-1091)
UniProt IDA3D286
Gene NameglgB; Locus:Sbal_1331
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-743
Expression Tag6xHis
Purity>90%
AA SequenceMMTQANAYFYDGADVALLNGQYTDVFSLLGMHSANEGKALIVRCFLRNAISVDVISIKDG RKVASLDKVNEQGLFAGTLGRRVKPFLYLLRVEYPLCQLDIVDPYQFDSLLNSDDIYLFG EGSAERAYEFLGANWRQTQGVEGVHFCVWAPNAKRVSVVGDFNHWDDTRHVMRQHLANGL WELFLPNVVEGAHYKFDLVYQNGERHTKSDPMATQMECAPHNASIVPPKAHHSWNDTAWM SKRAATAWHKAPMSAYEVHLGSWRRKGEQGEQYLDYQDLIEQLIPYVKEQGFTHIELMPI SEFPFDGSWGYQPVGLYAPTHRFGDANGLKAFVDACHQAGIGIILDWVSAHFPKDPHGLV RFDGTCLYEHEDPRKGTHPDWDTLIYNYDRGEVRSFLLSNACYWLREFHFDGLRLDAVSS MLYLDYSREPGQWLPNAYGGRENLEAISFLQILNQRLYQAFPGICMIAEESTAFAGVTKP TDQQGLGFGFKWNMGWMNDSLSYLGRDPLYRQFHHHQLTFSLMYAYTEQFMLSVSHDEVV HGKGSLLHKIPGDDWQKFATLRAYYGFMWGHPGKKLLFMGCEFGQRNEWNHNQSLDWHLL AYEPHQGVQRWLKDLNHLYQAMPALSVQDYEGAGFSWLDCENSRDSIFTFVRYGLAGDAP LVFVINMTPQLHTGFRIGLPLAGDYREYLNSDSQIYGGSNQGNAGTVVAESLPWQGMAQS ALITVPPLGCLVIGPATGLAEAN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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