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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP857255RCUYeast1mgQuote
EP857255RCUE. coli1mgQuote
BP857255RCUBaculovirus200ugQuote
MP857255RCUMammalian Cell200ugQuote

Protein Information

SpeciesRhizobium loti (strain MAFF303099) (Mesorhizobium loti)
UniProt IDQ985P4
Gene NameglgB; Locus:mlr7587
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-737
Expression Tag6xHis
Purity>90%
AA SequenceMRKPRVTAATSGPDGLAPASDVAAIVAGTHGDPFAVLGVHEVGKGLFARCFVPHAETVTA YTLTGIEAGALSRRDDAGFFEGKLSIKKRQPLRYHAHNAGGDWWLTDPYSFGPVLGPMDD YYIAEGSHLRLFDKLGAHVIEHEGATGVHFAVWAPNAKRVSVVGDFNDWDGRRHTMRDRR DTGIWEVFIPDIGAGRPYKYEIIGPDGVRLPLKADPFAFKSELRPATASVVAVPPAHDWG DEAHRNYWRNADPRREAVSIYEVHAGSWQLHDDGTFLSWDELADRLIPYVVETGFTHIEF MPISEHPYDPSWGYQTTGLYAPSARFGDPDGFARFVDGAHRAGVGVILDWVPAHFPVDAH GLAHFDGTALYEHADPRKGFHPDWNTAIYNFGRREVVSFLVNNALFWAEKYHVDGLRVDA VASMLYLDYSRKAGEWIPNEKGGRENLEAVSFLQKMNKEVYGHHPGVMTIAEESTSWPKV SAPVHEGGLGFGFKWNMGFMHDTLEYFSKEPIFRKHHHNDLTFGLTYAFSENFVLPLSHD EVVHGKGTLLSKMAGDDWQKFATLRAYYGFMWGYPGKKLLFMGQEFAQRREWSEARALDW NLLDFRPHRGVWQTVRDLNYLYRSRPALHGRDCEPEGFSWLIVDDSQNSVFAWVRNAPGG SPVAVISNFTPVPRDNYRVPLPKAGKWREIINTDASEYGGSGMGNGGMVEARAEGKNISA TMLLPPLSTIMLELVAD
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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