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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP765469MLAYeast1mgQuote
EP765469MLAE. coli1mgQuote
BP765469MLABaculovirus200ugQuote
MP765469MLAMammalian Cell200ugQuote

Protein Information

SpeciesMycobacterium paratuberculosis
UniProt IDQ73X75
Gene NameglgB; Locus:MAP_2434
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-731
Expression Tag6xHis
Purity>90%
AA SequenceMSHADQLARTHLAPDPADLSRLVAGTHHDPHGILGAHEYGDHTVIRAFRPHATEVAALVG GDRFAMQHIESGLFAVALPFTNLIDYRLQITYPDSEPYVVADAYRFLPTLGEVDLHLFGE GRHERLWEVLGAHPRSFTTADGVVTGVSFAVWAPNAKGISLIGEFNGWTGTEAPMRVLGS SGVWELFWPDFPIGGLYKFKVHGADGVVTERADPMAFATEVPPHTASRVTLSSYTWGDAD WMTQRAARNPVFEPMSTYEVHLGSWRPGLSYRQLARELTDYVVEHGFTHVELLPVAEHPF AGSWGYQVTSYYAPTSRFGTPDEFRALVDALHQAGIGVIVDWVPAHFPKDAWALGRFDGT PLYEHSDPKRGEQLDWGTYVFDFGRREVRNFLVANALYWLQEFHIDGLRVDAVASMLYLD YSRPEGGWTPNIYGGRENLEAVQFLQEMNATAHKSAPGIVTIAEESTSWPGVTRPTNLGG LGFSMKWNMGWMHDTLDYISRDPIYRSYHHHEMTFSMLYAFSENYVLPLSHDEVVHGKGT LWGRMPGNNHVKAAGLRSLLAYQWAHPGKQLLFMGQEFGQRAEWSEERGLDWWQLDEQGF SNGILRLVRDINDIYRSHPALWSQDTVPDGYSWIDANDSANNVLSFLRYGKDGSVMACVF NFAGAEHSGYRLGLPSAGRWREVLNTDATVYNGSGIGNMGGVDATEDPWHGRPASAVLVL PPTSALWLEPK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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