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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP672976Yeast1mgQuote
EP672976E. coli1mgQuote
BP672976Baculovirus200ugQuote
MP672976Mammalian Cell200ugQuote

Protein Information

SpeciesThermobifida fusca (strain YX)
UniProt IDQ47SE7
Gene NameglgB; Locus:Tfu_0582
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-749
Expression Tag6xHis
Purity>90%
AA SequenceMTARPAVRQPAGLPCPQPCNRYGYPMTNALLAEIDALVAGTHHNPHALLGAHPGPEGVWV RALRPLARSVHVLLANGSRVELPHLHKGVFAGVVPGAEVPDYRLVVRYDDGTELTVDDPY RHLPTLGELDIHLIQEGRHEELWRVLGAHTKRFPSVLGDTEGTAFTVWAPNARGVRVIGD FNHWDGTGHPMRSLGSCGVWELFIPGVGDGTRYKYQVLGADGVWREKADPVAFATQAPPE TASVVFTSRYTWQDDEWLTQRAAADLHRKPMSIYEVHLGSWRPGLSYRELADQLVDYVRE LGFTHVEFLPVAEHPFGGSWGYQVTSYYAPTARFGSPDDFRYLVDRLHQAGIGVFLDWVP AHFPKDDWALSRFDGTALYEHPDPRRGIHPDWDTLIFNYGRTEVRNFLVANALFWLEEFH IDGLRVDAVASMLYLDYSRESGQWEPNAYGGRENLDAIDFLRELNATAYRRNPGIAMIAE ESTAWPGVTRSTDTGGLGFGFKWNMGWMHDTLSYLQHDPVHRQYHHNEVTFSMVYAYSEN YVLPLSHDEVVHGKRSLLYKMPGNEWQRCANLRALLAYMWAHPGKQLLFMGNEIAQGDEW SHDAGVQWWLLRYPHHAGMRRLVADLNRLYRNTRALWSQDTVPEGFTWLDGGDASGNTLS FLRWGDDGSVLACLVNFSGRPHPERRVGLPYAGRWREILNTDAVLYGGSGVSQPGIIEAS EETPWQGQPASALVTYPPLGVSWLVFDGT
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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