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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP669580Yeast1mgQuote
EP669580E. coli1mgQuote
BP669580Baculovirus200ugQuote
MP669580Mammalian Cell200ugQuote

Protein Information

SpeciesDechloromonas aromatica (strain RCB)
UniProt IDQ47II8
Gene NameglgB; Locus:Daro_0586
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-621
Expression Tag6xHis
Purity>90%
AA SequenceMQHADALYLFNEGRNNQAYRLLGAHADISGTTFRVWAPNASRVSVVGDFNGWHGDVHQLH PLGESGVWEIAVAEAHTGNLYRFEITNRHTGDKLIKSDPYGRGFELRPGSAAYVVPPSMH VWGDADWLQQRAGWDWQQAPVNIYEVHPGSWMRHPDGKPYLWGELAERLIPYALAQGYTH LELLPITEHPLDESWGYQTTGYFAPTSRYGSADELRAFVDACHQAGLGVLLDWVPGHFPQ DDWALARFDGTALYEHEDPRLGLHADWGTHIFNYGRHEVRSFLMSSAHWWLSEFHFDGLR VDAVASMLYLDYSRKHGEWLPNKFGGRENLEAIDFLKQLNAMVHGDFPGALTIAEESTAW PMVSRPTYVGGLGFSMKWNMGWMNDSLRYFHRDPIYRRWHHSELTFGQIYAYSENYVLPF SHDEVVHGKGSLLGKMPGDTWQRYANLRLLLAWQVLTPGKKLMFMGCEFGQQGEWSEGRE LDWGSLANPENAAAQRLSADLNRLYRDLPALHTQDFSALGFEWIDCNDSEHSVLSWLRWG KDGSFVVVVFNFTPVPQPAYRLGVPEAGTYVELLNTDSAFYGGSNLGNGGALTATKGEWM GRPANLEVTIPPLGAVVLRRE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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