Request QuoteCatalog Number: xP670587DZRSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP670587DZRYeast1mgQuote
EP670587DZRE. coli1mgQuote
BP670587DZRBaculovirus200ugQuote
MP670587DZRMammalian Cell200ugQuote

Protein Information

SpeciesCupriavidus pinatubonensis (strain JMP134 / LMG 1197) (Alcaligenes eutrophus) (Ralstonia eutropha)
UniProt IDQ46TF2
Gene NameglgB; Locus:Reut_B4229
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-750
Expression Tag6xHis
Purity>90%
AA SequenceMNRVDTRPPERRTQTISDGELGALVEGRHRDPFAILGPHRDGDTLIVRACVPGAHSVMLA DSRGEPLAPMTPLHAGGVFTGRLPAGVSVYQLLVRWHNGTQQVSHDPYAFGLLLGELDLH LIAEGRHFELGACLGAQWRNVDGVQGVRFAVWAPNARRVSVIGDFNGWQPARHPMRLRHP SGVWELFIPEAMGARPGCRYKFDLLDPHDAQLPDKADPLALATEAPPATASVVTQPQVSA PPFAWQDDEWMRLRNAVDPYAAPLSIYEVHVGSWLRAANDPARGWEVLADRLIPYVHELG FTHIELLPVTEHPFGGSWGYQPLSLYAPTARLGPPQAFAAFIDRCHRDGIGVILDWVPAH FPTDPHGLARFDGTALYEHEDPREGFHQDWNTLIYNLGRNEVRGFLLAGALHWLEHFHVD GLRVDAVASMLYRDYSRAPDQWVPNRFGGRENLEAVAFLRELNTVVHERCPGALTIAEES TAWPGVTASAASGGLGFDFKWNMGWMHDTLRYLSLDPIHRAWHHQDMTFGTVYAWSEAFV LPLSHDEVVHGKGSMLRKCPGDDWQRFAGLRAYYGFMWAHPGKKLLFMGGELAQWQEWNH DAELDWALLDHPMHRGVHTLVRDLNALYRELPALHELDHSPAGFQWVVGDDHQNSVFAWL RRPAPGSGDVVLAVTNMTPVPRYGYRIGVPSEGCWHERLNTDAACYGGSNLGNGGAVTAE PVPSHGQEASVLLTLPPLATVILQHAGTQA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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