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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP670582Yeast1mgQuote
EP670582E. coli1mgQuote
BP670582Baculovirus200ugQuote
MP670582Mammalian Cell200ugQuote

Protein Information

SpeciesProchlorococcus marinus (strain NATL2A)
UniProt IDQ46LW6
Gene NameglgB; Locus:PMN2A_0020
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-755
Expression Tag6xHis
Purity>90%
AA SequenceMTVSILLDSLKDDGQRLSECRHESPFSILGPQPFKDKWIIRIWMPEASQVELVTQQTKIK LQNPNHEWIFEGVLEKDPGTDYQIKVNRGGIEHVQHDPWSFRKEWMGEIDRHLFAEGNHH HIWRKMGAHLTEIDKKKGVMFCLWAPHAKSVSVIGDLNSWDGRHHPMQKRLGGIWELFIP GLSEGDLYKYEIRTEKGHCYEKADPYGFQHEVRPAKSSVISKIDSFQWSDQSWISNRDNR DPLEQPISVYEMHLGSWMHASSDDPFINSNGEHRAPVPAADMKPGSRLLTYKELANKVIP YVKERGFTHIELMPISEHPFDGSWGYQVTGWYAPTSRFGSPDEFRAFVDSCHKEGIGIIL DWVPGHFPKDQHGLAYFDGSHLYEHSDPRVGEHKEWGTLIFNYSRNEVRNFLVANLIFWF DQFHIDGIRVDAVASMLYKDYLRPEGEWIPNEDGGNENFEAVRFLQQANHVLFQHFPGAL SIAEESTTWTGVTKPTDMDGLGFNLKWNMGWMHDMLDYFEIDPWFRQFNQNNITFSICYN FTENFMLALSHDEVVHGKSHLLHKMPGDDWQKYANTRALLAYMWTHPGKKTIFMGMEFGQ RQEWNVWDDLQWDLLNYEPHKGIQKLVDDLNNLYKREPALWRNDFDEYGFQWIDCDDNKN SVISFMRREKTDGEWLVIVANFTPQNHSNYRIGVPVDGFYEEIFNTDASQYGGSNLGNMG GKSTDLYNIHGYENSIDLCLPPLSVLVLKHKSKKN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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