Request QuoteCatalog Number: xP710714VFBSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP710714VFBYeast1mgQuote
EP710714VFBE. coli1mgQuote
BP710714VFBBaculovirus200ugQuote
MP710714VFBMammalian Cell200ugQuote

Protein Information

SpeciesVibrio fischeri (strain ATCC 700601 / ES114)
UniProt IDQ5DZB8
Gene NameglgB; Locus:VF_A0808
Protein Name1,4-alpha-glucan branching enzyme GlgB
Region Expressed1-715
Expression Tag6xHis
Purity>90%
AA SequenceMQLERAAFSDPFSFLGPQYQSQTTALRVWLPGATSVKVRLSGHVEYQLLSDPRHSGIFVL NESIDMTEVHYELIIDWSGTEQILDDPYQYHDISPTDAQVHTPKEMYNHLGAHLFNVVRD GKQIQGVRYLVFAPNASSASVIGDFNQWDGRRHIMQRIDNGLWALFIPEHAVGTKYKFEL KGPNGESLPHKMDPYGAHNEQYPSFASVVYDQTSYQWNDAKWQQRPVTEKQKEALSFYEL HAGSWKRNENGDFLTYRELAEQLIPYILDMGYTHIELMPVSEHPFYGSWGYQPIGLFSPT SRFGTPDDFKYFVDQCHQVGIGVVLDWVPAHFPSDSHGLANFDGTSLFNDPDPRRGWHND WQSFIYNYDQPHVREFLVSNALYWFEHFHIDGLRVDAVASMLYLDYSRNDGEWIPNWEGG NHNHGAIALLKWMNEEVYSHYPNAMTIAEESTAFPGVSAPTFAGGLGFGFKWNMGWMHDS LNYIREDPIHRKYHHDTITFPLVYAFSENFILSLSHDEVVYGKGSILDKMPGDEWQKTAN LRAYMGYMYGQPGKKLNFMGAEIAQSAEWDHDGQLQWFLTQFERHSGMQSLVRDLNKLYT TEPALYQKDCEPAGFEWRLQDEAEMSVLAHERLGDNGERILVVSNFTPAPREDFRLGMPV AGQYELILNSDAHFYGGSDYSVISEASTEKVESQGLAQSIVITLPPLSTVFYRLK
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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