Request QuoteCatalog Number: xP453444DQYSize: 0.2-1mg

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Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB)

Recombinant 1,4-alpha-glucan branching enzyme GlgB (glgB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP453444DQYYeast1mgQuote
EP453444DQYE. coli1mgQuote
BP453444DQYBaculovirus200ugQuote
MP453444DQYMammalian Cell200ugQuote

Protein Information

SpeciesCellvibrio japonicus (strain Ueda107) (Pseudomonas fluorescens subsp. cellulosa)
UniProt IDB3PGN4
Gene NameglgB; Locus:CJA_1885
Protein Name
Region Expressed1-737
Expression Tag6xHis
Purity>90%
AA SequenceMSNPKQEQHLSQDLLRIITATHHDPFEVLGRHSLPVPTATADTLVRVYLPGARSAELVIN RQNRQIAPLNRLPGTDFFEWYGLGQQLPVHYQIEWYDRHNQHRVHYDPYAFAPQLGELDM HLFAEGQHWNIYNLLGAHPRQVDGINGVLFATWAPNAERISVVGNFNDWDGRHHPMRVRG SSGLWELFIPGVQPGDLYKFEIRNRQTGAVFLKSDPYGQSFEHRPSTSSIITADSAFAWQ DADWMEHRAHWDWQASPLSIYEVHLGSWRRGWAGEFLSYRELAHQLADYVKYMGFTHVEI LPVSEHPLDDSWGYQTTGYYAPTSRFGSPDDFRYFVDHLHQQGIGIILDWVPAHFPKDAH ALARFDGSALYEHEDPRLGEHRDWGTLIYNYGRSEVRNFLLANALFWLKEYHIDGLRVDA VASMLHLDYSRQPGEWIPNIHGGNENLEAMTFLQQLNAICHGQHSGALVIAEESTAWPQV TRPTWVGGLGFSMKWNMGWMHDTLEYMSREPVHRQYHHNQLTFGMMYAFTENFQLPFSHD EVVHGKGSMINKMPGDDWQKFANLRLLYTYLYTYPGSKLLFMGGEFAQWSEWAHGRSLDW HLLDYGPHQGVQSAVKDLNHLYRQVPSLYQRNFRGDGFEWIDCHDSTQSIISYIRSSDSE FTLVILNFTPVPRYNYRIGVPVAGQYQEVFNSDSQFYGGSNLGNANPLYTQPVPWMNHGQ SLEVTLPPLGGLLLKLA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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