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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (LMJF_20_0970)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (LMJF_20_0970) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP709001LOGYeast1mgQuote
EP709001LOGE. coli1mgQuote
BP709001LOGBaculovirus200ugQuote
MP709001LOGMammalian Cell200ugQuote

Protein Information

SpeciesLeishmania major
UniProt IDQ4QCU9
Gene NameORFs:LMJF_20_0970
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-344
Expression Tag6xHis
Purity>90%
AA SequenceMTDCWYIPEAVADRRDENRLSPNVPASYEVLGEAGIFYRHFDSEEVNDNIEGFIQPLLKK LNYQSYDVVNLSPANLGAEKFETLAEQHFMEHIHEDDEVRLILEGQGYFDVRDINDKWIR LLLKPGDCIVVPAGMYHRFTTDQSKCIKTLRIFKEAPQWIALNRGPEAEEKPARKEYLAR LHAPAETAVGAVNGRTIFSLRYPLKLDAELTVITKRLLEQHSKQPLALAIYLTGSTDPTT GESWCPDCVLAKLHVARRFAELQGTYGKEHAIFLQLPVERASYLGNPNFFYRTHPILQLA SVPTLLVLSPAKDAKEGGDVQWYDLLDVKVRTCDVDRTDVLNLE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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