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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (adi1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (adi1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP709316NGSYeast1mgQuote
EP709316NGSE. coli1mgQuote
BP709316NGSBaculovirus200ugQuote
MP709316NGSMammalian Cell200ugQuote

Protein Information

SpeciesNeosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) (Aspergillus fumigatus)
UniProt IDQ4WDE1
Gene Nameadi1; ORFs:AFUA_6G04430
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-176
Expression Tag6xHis
Purity>90%
AA SequenceMKAYWYDNKPGDQREPHDSGRPVTVDYLASIGVQYYHFPSLESVNELAKERGYKNRDEIV VSPQAMGDVYEEKVKMFFNEHLHEDEEIRYIRDGEGYFDVRGQEDEWVRIKLAKDDLIIL PAGIYHRFTTDDKNYVKAMRLFQEEPKWTPLNRGPDVDENSHRQSYLRTIQNKTVA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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