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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP605710EUGYeast1mgQuote
EP605710EUGE. coli1mgQuote
BP605710EUGBaculovirus200ugQuote
MP605710EUGMammalian Cell200ugQuote

Protein Information

SpeciesPhaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) (Glume blotch fungus) (Septoria nodorum)
UniProt IDQ0UG91
Gene NameADI1; ORFs:SNOG_09223
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-175
Expression Tag6xHis
Purity>90%
AA SequenceMRAYWFDNLEGDQRQPHDSGRAVDPAYLSKLGVLYHHISSQSEVDELAKARDYKNRDEIT VSPEKMGDIYEEKVKSFFHEHLHEDEEIRYILDGAGYFDVRSEGDDWVRIWLEKGDLIIL PSGIYHRFTTDEQNYTKAMRLFKDEPKWTPLNRGEETDENQFRQEYLKLRQGLAA
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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