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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (adi1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (adi1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP528411SXVYeast1mgQuote
EP528411SXVE. coli1mgQuote
BP528411SXVBaculovirus200ugQuote
MP528411SXVMammalian Cell200ugQuote

Protein Information

SpeciesSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
UniProt IDO94286
Gene Nameadi1; ORFs:SPBC887.01
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-178
Expression Tag6xHis
Purity>90%
AA SequenceMRAYIFQDEGDQRKPNDSKIEVSAEDLEAAKVSYRHHDGDLHTFADGLMKEYGFKNRDEV VVSRKGLGDRYDNMVKKFFEEHLHEDEEIRLILDGNGYFDVRSVDDRWVRIFVEKGDLII LPPGIYHRFTTTTDDYIHAMRLFHENPKWIALSRTDSTSEELDARKSYLNSIKKSVYV
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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