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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP518975MRMYeast1mgQuote
EP518975MRME. coli1mgQuote
BP518975MRMBaculovirus200ugQuote
MP518975MRMMammalian Cell200ugQuote

Protein Information

SpeciesMetarhizium robertsii (strain ARSEF 23 / ATCC MYA-3075) (Metarhizium anisopliae)
UniProt IDE9EUE8
Gene NameADI1; ORFs:MAA_03647
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-151
Expression Tag6xHis
Purity>90%
AA SequenceMYDNQPRLPHDSGRAISTEALGKLGVLYFHFANIADVDRLAADRGYKNRDEITVSPEKMG GMYEDKVKMFFNEHLHEDEEIRYIKGGQGFFDVRSKDDNWVRVRLEKDDLLILPAGIYHR FTTDEANVPVPRIVRTCHEALQGRAQMDAIE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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