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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (LBRM_20_5150)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (LBRM_20_5150) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP516790LOCYeast1mgQuote
EP516790LOCE. coli1mgQuote
BP516790LOCBaculovirus200ugQuote
MP516790LOCMammalian Cell200ugQuote

Protein Information

SpeciesLeishmania braziliensis
UniProt IDE9AIE8
Gene NameORFs:LBRM_20_5150
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-344
Expression Tag6xHis
Purity>90%
AA SequenceMTECWYIPEEVADRREENRLSPNVPGSYEALSEARVFFRHFDPKEVSDDIEGFIQPLLKK LNYHSYDVVNLSPATLGDEKFEALAEQHFMEHTHEDDEVRLILEGQGYFDVRDANDKWIR LLSKPGDCIVLPAGMYHRFTTDHSKCIKTLRIFKEAPRWIALNRGPETEEKPARKEYLAR LHAPAETAVGAANSHTIFSLHYPLKLDAELTVITKRLLEQHSKQPLALMIFLTGSTDPTT GASWCPDCIPAKPQVAQRFAELQGKYGEERAIFLQLPVERAGYLGNPEYPYRKHPTLQLA SVPTLLVLTPTKDAKEKGDVQWYDRLEVKMRTCDIDKADVLSLE
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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