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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (ADI1) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP514111EXOYeast1mgQuote
EP514111EXOE. coli1mgQuote
BP514111EXOBaculovirus200ugQuote
MP514111EXOMammalian Cell200ugQuote

Protein Information

SpeciesPolysphondylium pallidum (Cellular slime mold)
UniProt IDD3BH90
Gene NameADI1
Protein Name1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase
Region Expressed1-146
Expression Tag6xHis
Purity>90%
AA SequenceMKSYYFESNEPVSVQQLNNINVLYYPMPMPDYQEKLDQICQERGYKNRDEVKLNENTENL DAKLKIFFEEHLHDDEEIRFILEGSGFFDVRDANDQWIRIRVEKGDLIIVPANMYHRFTL TETKQIHACRLFTDAPKWVPINRHQQ
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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