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Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (12562)

Recombinant 1,2-dihydroxy-3-keto-5-methylthiopentene dioxygenase (12562) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP435886MODYeast1mgQuote
EP435886MODE. coli1mgQuote
BP435886MODBaculovirus200ugQuote
MP435886MODMammalian Cell200ugQuote

Protein Information

SpeciesMonosiga brevicollis (Choanoflagellate)
UniProt IDA9VCM7
Gene NameORFs:12562
Protein Name
Region Expressed1-176
Expression Tag6xHis
Purity>90%
AA SequenceMKSWYMADVPEGGDPREPYMTDPLQEVSAEQLKALGVLYWHIPVEDHEEKLKAVCDERNY AAQDMITCSREKLPNYEDKLKTFFTEHLHEDEEIRYVTAGTGYFDVRSKDDRWIRIQVQP GDLLILPSGIYHRFTLDTDNYIQAKRLFQAEPKWTPINRPADDNSFRVEYLKALQN
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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