Request QuoteCatalog Number: xP501925VEZSize: 0.2-1mg

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Recombinant Vitamin B12 transporter BtuB (btuB)

Recombinant Vitamin B12 transporter BtuB (btuB) can be expressed and purified from different hosts. E. coli and yeast offers the best yields and shorter turnaround times. Expression in insect cells with baculovirus or mammalian cells can provide many of the posttranslational modifications necessary for correct protein folding or retain the proteins activity.

Catalog NumberExpression HostAmountIn Stock
YP501925VEZYeast1mgQuote
EP501925VEZE. coli1mgQuote
BP501925VEZBaculovirus200ugQuote
MP501925VEZMammalian Cell200ugQuote

Protein Information

SpeciesVibrio cholerae serotype O1 (strain M66-2)
UniProt IDC3LPZ6
Gene NamebtuB; Locus:VCM66_0155
Protein NameVitamin B12 transporter BtuB
Region Expressed23-611
Expression Tag6xHis
Purity>90%
AA SequenceNEAQPQETVVVTANRFEQKASSTLADVEIITRQDIEQTQAKTLPELLRRLTGVQITQNGG RGQLASLFVRGTSSDQVLVLVDGIRFARAAKGAVDFNQIPLTYVDRIEYVRGARASLYGS EAIGGVINIITKARSQQQGTTVSAGLGSLDYQELSIASGVAIGEKGQMNVALGTESDKGY NVRPVPGVNDGDRHGFRSDNALLGYVHQFDESWSLFANARAYENIYQYDNSYGTRDYKEA EKDDLSFTIGTQYQSERWVSELQLTTQKQKSWDYTQSKGKYSDTSDNLEQRNIQWINRYL VNDVWTFAGGVDWRDESYIDKTADKEFDRSNTAAFAVVAAEWQQWLLEASLRFDDNQEYG SQTTHNIALGYQFIPEFGVKASYGSAFKAPNLYQQYDPSYGNVNLQPEDADSAELSFYGL FSGIKWSITGYDYKINNLIDYNSTTKNYQNVIGESNIKGVEFTAEFATGIVQHQLSVDLK DADDSKGKTLQRRAEHMYKWNALVAFEQVDWSIGYQYVGKRPDLDYNTYPTQNITLDAYS LVDTSVSYYVTDSTTISARIDNLLDKEYETANGYPAAERAYYLNIGYQF
Storage BufferPBS pH 7.4, 50% glycerol
StorageStore working aliquots at 4oC for upto a week. Freeze at -20 to -80oC for long term storage. Avoid repeated freeze/thaw.
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