$90.00Catalog Number: PYT318-5ugSize: 5ug

Visfatin Human

Visfatin Human Recombinant

Visfatin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 348 amino acids and having 11 N-terminal flag tag The total molecular mass is 39.6 kDa (calculated). The amino acid sequence of the recombinant human Visfatin is 100% homologous to the amino acid sequence Pro27-Glu363 of the human Visfatin.
The Visfatin is purified by Flag-affinity chromatography.

Accession

P43490

Amino acid sequence

MDYKDDDDKA SPPNTSKVYS YFECREKKTE NSKLRKVKYE ETVFYGLQYI LNKYLKGKVV TKEKIQEAKD VYKEHFQDDV FNEKGWNYIL EKYDGHLPIE IKAVPEGFVI PRGNVLFTVE NTDPECYWLT NWIETILVQS WYPITVATNS REQKKILAKY LLETSGNLDG LEYKLHDFGY RGVSSQETAG IGASAHLVNF KGTDTVAGLA LIKKYYGTKD PVPGYSVPAA EHSTITAWGK DHEKDAFEHI VTQFSSVPVS VVSDSYDIYN ACEKIWGEDL RHLIVSRSTQ APLIIRPDSG NPLDTVLKVL EILGKKFPVT ENSKGYKLLP PYLRVIQGDG VDINTLQE.

Applications

Western Blot.

Formulation

Filtered (0.4 um) and lyophilized from 0.5 mg/ml in 20mM Tris, 20mM NaCl, pH 7.5.

Physical Appearance

Filtered White lyophilized (freeze-dried) powder.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Solubility

Add 20mM Tris, 20mM NaCl, pH 7.5 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Source

Escherichia Coli.

Stability

Lyophilized Visfatin although stable at room temperature for 3 weeks, should be stored desiccated below -18oC. Upon reconstitution Visfatin should be stored at 4oC between 2-7 days and for future use below
-18oC.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Synonyms

PBEF, Pre-B cell colony-enhancing factor, Nicotinamide phosphoribosyltransferase NAmPRTase, Nampt, MGC117256, DKFZP666B131, 1110035O14Rik.

Quantity

$90.00

ReviewsWrite Review